H&F presses on with $4 billion Getty Images sale: sources

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Source: http://news.yahoo.com/h-f-presses-4-billion-getty-images-sale-010652549--sector.html

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Making Raindrop Glare Disappear Using Smart Headlights [Automobiles]

You're driving down a twisting mountain road at night and all of a sudden the storm clouds open. Whatever you do with your headlights you can barely see more than a couple of feet. Fortunately, that's all set to change thanks to a team of scientists who can make the reflections from the rain disappear. More »

Source: http://feeds.gawker.com/~r/gizmodo/full/~3/JD_OGsW_v5k/making-raindrop-glare-disappear-using-smart-headlights

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Scientists develop alternative to gene therapy

ScienceDaily (July 1, 2012) ? Scientists at The Scripps Research Institute have discovered a surprisingly simple and safe method to disrupt specific genes within cells. The scientists highlighted the medical potential of the new technique by demonstrating its use as a safer alternative to an experimental gene therapy against HIV infection.

"We showed that we can modify the genomes of cells without the troubles that have long been linked to traditional gene therapy techniques," said the study's senior author Carlos F. Barbas III, who is the Janet and Keith Kellogg II Professor of Molecular Biology and Chemistry at The Scripps Research Institute.

The new technique, reported in Nature Methods on July 1, 2012, employs zinc finger nuclease (ZFN) proteins, which can bind and cut DNA at precisely defined locations in the genome. ZFNs are coming into widespread use in scientific experiments and potential disease treatments, but typically are delivered into cells using potentially risky gene therapy methods.

The Scripps Research scientists simply added ZFN proteins directly to cells in a lab dish and found that the proteins crossed into the cells and performed their gene-cutting functions with high efficiency and minimal collateral damage.

"This work removes a major bottleneck in the efficient use of ZFN proteins as a gene therapy tool in humans," said Michael K. Reddy, who oversees transcription mechanism grants at the National Institutes of Health's (NIH) National Institute of General Medical Sciences, which helped fund the work, along with an NIH Director's Pioneer Award.

Questioning Assumptions

ZFNs, invented in the mid-1990s, are artificial constructs made of two types of protein: a "zinc-finger" structure that can be designed to bind to a specific short DNA sequence, and a nuclease enzyme that will cut DNA at that binding site in a way that cells can't repair easily. The original technology to make designer zinc finger proteins that are used to direct nucleases to their target genes was first invented by Barbas in the early 1990s.

Scientists had assumed that ZFN proteins cannot cross cell membranes, so the standard ZFN delivery method has been a gene-therapy technique employing a relatively harmless virus to carry a designer ZFN gene into cells. Once inside, the ZFN gene starts producing ZFN proteins, which seek and destroy their target gene within the cellular DNA.

One risk of the gene-therapy approach is that viral DNA -- even if the virus is not a retrovirus -- may end up being incorporated randomly into cellular DNA, disrupting a valuable gene such as a tumor-suppressor gene. Another risk with this delivery method is that ZFN genes will end up producing too many ZFN proteins, resulting in a high number of "off-target" DNA cuts. "The viral delivery approach involves a lot of off-target damage," said Barbas.

In the new study, Barbas and his colleagues set out to find a safer ZFN delivery method that didn't involve the introduction of viruses or other genetic material into cells. They experimented initially with ZFN proteins that carry extra protein segments to help them penetrate cell membranes, but found these modified ZFNs hard to produce in useful quantities. Eventually, the scientists recognized that the zinc-finger segments of ordinary ZFNs have properties that might enable the proteins to get through cell membranes on their own.

"We tried working with unmodified ZFNs, and lo and behold, they were easy to produce and entered cells quite efficiently," Barbas said.

New Strategy Against HIV

Next, the team showed how the new technique could be used in a ZFN-based strategy against HIV infection.

The AIDS-causing retrovirus normally infects T cells via a T cell surface receptor called CCR5, and removing this receptor makes T cells highly resistant to HIV infection. In 2006, an HIV patient in Berlin lost all signs of infection soon after receiving a bone marrow transplant to treat his leukemia from a donor with a CCR5 gene variant that results in low expression of the receptor. Disrupting the CCR5 gene in T cells with a ZFN-based therapy might be able to reproduce this dramatic effect.

"The idea is to protect some of the patient's T cells from HIV, so that the immune system remains strong enough ultimately to wipe out the infection," said Barbas.

A gene therapy that uses ZFNs to disrupt CCR5 genes in T cells and reinfuses the modified T cells into patients is currently in clinical trials. Barbas and his team showed that they could achieve the same effect with their simpler ZFN-delivery method. They added ZFN proteins directly to human T cells in a culture dish and found that within hours, a significant fraction of the ZFN-treated cells showed sharp reductions in CCR5 gene activity.

After several applications of ZFNs, aided by a special cooling method that improves the ability of the proteins to get across cell membranes, the scientists were able to inactivate CCR5 genes with an efficiency approximating that of the gene therapy-based approach, Barbas said.

The new approach also appeared to be safer. A DNA-based method the team used for comparison or the viral-based methods reported in the literature by others ended up producing ZFNs for up to several days, causing a significant amount of off-target DNA damage. But the directly delivered ZFN proteins remained intact within cells for only a few hours, causing minimal off-target damage.

"At some off-target locations where the gene therapy approach frequently causes damage, we saw no damage at all from this new technique," said Barbas.

Hope for 'Tiny Factories' of Health

The team tested its direct ZFN-delivery technique with a variety of other cell types and found that it works with particularly high efficiency in human skin "fibroblast" cells. Researchers now are working on advanced therapies in which they harvest such fibroblasts from patients and reprogram the cells' gene-expression patterns so that they effectively become stem cells. These induced stem cells can then be modified using ZFNs and other genome-editing techniques. When reinfused into a patient, they can produce millions of therapeutic progeny cells over long periods.

Such techniques may one day be used to treat a vast array of diseases. Barbas, who has been developing anti-CCR5 strategies for more than a decade, wants to start with a ZFN-based therapy that disrupts the CCR5 gene in hematopoietic stem cells. These blood-cell-making stem cells, reinfused into an HIV patient, would become tiny factories for producing HIV-resistant T cells.

"Even a small number of stem cells that carry this HIV-resistance feature could end up completely replacing a patient's original and vulnerable T cell population," he said.

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The above story is reprinted from materials provided by Scripps Research Institute, via EurekAlert!, a service of AAAS.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.

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Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/~3/eXa8XH5P_4k/120701191613.htm

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Olympic fever revs up fitness routines

NEW YORK (Reuters) - With the 2012 London Olympics just weeks away, weekend warriors, fitness fanatics and couch potatoes alike will soon be inspired to rev up their routines.

The fitness industry is gearing up for Olympic fever with workouts that reflect the athleticism, competition and teamwork of the games.

"Something about the Olympics makes people want to compete," said Samantha Clayton, a Malibu, California-based personal trainer and track coach. "The athletes make it look so easy that everyone gets that I-can-do-it feeling, even if they can't."

Clayton, who competed as a sprinter in the 2000 Sydney Olympics, has watched Olympic fever grip Pepperdine University, in Malibu, where she is head women's sprint coach.

"Around campus, it just inspires students to train hard and work harder, even if they aren't that caliber," she said.

A native of Birmingham, England, Clayton is particularly excited about the Olympics, which will begin in London on July 27.

"My entire family has a severe case of Olympic fever," she said. "A lot of my friends are still competing."

Clayton thinks much of the appeal lies in watching the many televised sagas of struggle and determination of individual Olympians, whom she describes as often otherwise ordinary people.

"It's a raw sort of sport. There's nothing fancy about it," she said. "A lot of the competitors hold down regular jobs."

Some motivation comes from having a goal, she said.

"Any training becomes easier when you have a purpose," she said, whether it's winning a gold medal or fitting into a wedding dress, and even a trainer wants something to work for.

She said people who don't care about sports still love the Olympics, and it only comes around every four years.

"You'll always have that one crazy person who thinks he can run 800 meters and pops a hamstring," she said. "But I think people are more inspired than disappointed."

NATURAL ATHLETIC EVENT

Lashaun Dale, national creative manager of group fitness at Equinox, said programming at the chain of fitness centers tends to be more athletically and sports inspired in Olympic years.

"The Olympics provide a natural athletic event for organizing programs around," said Dale. "It gives a little structure and bit more intensity."

She said one group fitness class, called Core Values, is inspired in part by gymnastic feats on the parallel bars, beam, vault and floor. Another class uses a rowing machine and taps into the competitive team spirit of the games.

Equinox timed the launch of some classes to coincide with the Olympics, when clients would be most responsive, Dale said.

"Members are interested in these types of classes around athletic events," she said. "People get excited around training for a goal, even if they're not athletes."

For personal trainers the client conversations around Olympic time often turn to upping goals, gaining mastery and training like an athlete, she said.

Olympian feats of strength, speed and power may be out of reach for most of us, but the American Council on Exercise (ACE) has created Olympic-inspired workouts anyone can use to get fit.

"The workouts are themed around gymnastics, track and field, cycling and water sports," said Jessica Matthews, an ACE exercise physiologist.

Olympic gold medalist Dominique Dawes has endorsed the gymnastics workout, and Olympic gold medalist and current Olympic hopeful Allyson Felix has endorsed the track and field.

"Our workouts are built on the foundational principles of fitness," said Matthews, adding that the principles apply whether you aim for a gold medal or fewer pounds.

"You establish a goal. You have a regimen. You stick with it," she said. "You will succeed."

Clayton believes the commitment is all.

"Once you sign up for an event you become an athlete," she said. "The second you pay your fee, even if it's for a walk, you become an athlete. You can think like one. And thinking like an athlete is what I encourage my clients to do."

(Editing by Patricia Reaney)

Source: http://news.yahoo.com/olympic-fever-revs-fitness-routines-090036487--spt.html

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ICC staffers held in Libya released, fly to Italy

Australian lawyer Melinda Taylor, third from left, prepares to board a plane to Rotterdam after disembarking from Tripoli at Rome's Ciampino military airport after being released from Libya, Monday, July 2, 2012. Taylor is one of the four International Criminal Court staffers who had been held for nearly four weeks on allegations that they shared documents that could harm national security with Moammar Gadhafi's imprisoned son Seif al-Islam Gadhafi. (AP Photo/Riccardo De Luca)

Australian lawyer Melinda Taylor, third from left, prepares to board a plane to Rotterdam after disembarking from Tripoli at Rome's Ciampino military airport after being released from Libya, Monday, July 2, 2012. Taylor is one of the four International Criminal Court staffers who had been held for nearly four weeks on allegations that they shared documents that could harm national security with Moammar Gadhafi's imprisoned son Seif al-Islam Gadhafi. (AP Photo/Riccardo De Luca)

Australian lawyer Melinda Taylor, third from left, holding a white bag, prepares to board a plane to Rotterdam after disembarking from Tripoli at Rome's Ciampino military airport after being released from Libya, Monday, July 2, 2012. Taylor is one of the four International Criminal Court staffers who had been held for nearly four weeks on allegations that they shared documents that could harm national security with Moammar Gadhafi's imprisoned son Seif al-Islam Gadhafi. (AP Photo/Riccardo De Luca)

Australian lawyer Melinda Taylor, second from left, and President of the International Criminal Court (ICC) Sang-Hyun Song, of South Korea, prepare to board a plane to Rotterdam after disembarking from Tripoli at Rome's Ciampino military airport after being released from Libya, Monday, July 2, 2012. Taylor is one of the four International Criminal Court staffers who had been held for nearly four weeks on allegations that they shared documents that could harm national security with Moammar Gadhafi's imprisoned son Seif al-Islam Gadhafi. (AP Photo/Riccardo De Luca)

ZINTAN, Libya (AP) ? Libya released on Monday four International Criminal Court staffers who had been held for nearly four weeks on allegations that they shared documents that could harm national security with Moammar Gadhafi's imprisoned son Seif al-Islam Gadhafi.

As they were released, ICC President Sang-Hyun Song, a South Korean judge, apologized to the Libyan government and people for the incident and promised an investigation into the allegations. Song flew to Libya for the handover.

"The ICC is grateful to the Libyan authorities for their agreement today to release the Court's staff members so that they can be reunited with their families," Song said as the four were released.

A special flight arranged by the Italian government and carrying Song and the staffers landed at Rome's Ciampino airport late Monday. The staffers and Song took off for Rotterdam a few minutes later.

The staffers did not speak to the media.

"Well, I'm very happy to bring them all back to freedom," the court president told reporters.

"The Libyan government gave me their version of the investigation. We will do our own separately, so the results will be known after some weeks," Song added.

Seif al-Islam was the most senior member of the ousted Gadhafi regime to be captured in last year's civil war. Long viewed as a likely heir-apparent to his father, he faces charges by the ICC of crimes against humanity. He was captured by a militia in western Libya after his father was taken captive and then killed last October following more than 40 years as Libya's eccentric, authoritarian ruler.

Libya's new leadership accused Seif al-Islam of torturing and killing rebels, as well as other crimes.

His trial has been at the heart of a dispute between the ICC and the Libyan government. Libyan authorities have challenged the ICC's right to try Seif al-Islam, saying the international court is a tribunal of last resort, intended to try suspects from countries that cannot or will not prosecute them.

The court had expressed concern that Libya's judiciary is not yet ready to give Seif al-Islam a fair trial. The ICC judges ruled on June 1 that Libya doesn't have to hand over Seif al-Islam at least until a ruling on Tripoli's challenge.

Richard Dicker, international justice program director at Human Rights Watch, said the release of the ICC employees was "overdue," and that if Libya had any concern over their conduct, it should have submitted a complaint to the ICC.

"It is unacceptable that the ICC staff would be held for nearly a month when Libya had no right to do so," he said in an email to The Associated Press.

Amnesty International echoed the same sentiment and urged the ICC to investigate the legality of the detention and the allegations made by the Libyan authorities. It also questioned Tripoli's readiness to offer Gadhafi's son a fair trial.

"Not only has it denied them (the ICC staffers) their liberty and stopped them from performing their functions, but it has also undermined Seif al-Islam Gadhafi's right to an effective defense and delayed the ICC's decision on the Libyan authorities' recent application to bring him to trial in Libyan courts," said Amnesty International's Marek Marczynski.

He urged the ICC to review the impact of the detention and take measures to ensure Gadhafi's defense team has time to prepare their case.

The Libyan lawyer who represents Tripoli's interests at the ICC, Ahmed Al-Jehani, said the mission staffers were released because they have diplomatic immunity.

Libyan authorities say they placed Australian defense attorney Melinda Taylor and her Lebanese translator under house arrest after they visited Seif al-Islam in prison in the western town of Zintan and allegedly shared documents that could harm the country's national security. Seif al-Islam was captured by Zintani rebels late last year and has been held there ever since.

Al-Jehani said state prosecutors accused Taylor of having Seif al-Islam sign three blank pieces of paper, but they could not press charges because she has diplomatic immunity. Al-Jehani said Libyan authorities do not know why she wanted his signature.

Libyan Deputy Foreign Minister Mohammed Aziz said the staffers, which also include a Russia national and a Spanish national, will leave to Tripoli and then Italy later in the day.

Al-Jehani, who is also the Libyan prosecutor leading the case against Gadhafi's son, said they were in good health. He said the case has now been closed in Libya and the staffers will not return.

"Definitely they are free and they will not be coming back to Libya," he said.

Al-Jehani said the detention of the ICC staff has further complicated relations with Libya.

"The relationship has become more difficult and more unstable," he said, adding that Libya would likely press ahead with its case against Seif al-Islam after the country's first nationwide elections this week to choose a parliament.

___

Associated Press writers Aya Batrawy in Cairo, Frances D'Emilio and Pietro De Cristofaro in Rome and Mike Corder in The Hague, Netherlands, contributed to this report.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/cae69a7523db45408eeb2b3a98c0c9c5/Article_2012-07-02-Libya-ICC/id-699d7ff962b744499329c373da4a77b1

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Google Currents to be part of Jelly Bean OTA for Nexus devices

As you'll know if you've been following our Google I/O coverage, Google Currents will ship on the Nexus 7 tablet when it launches next month. But now it's been revealed that Google's digital magazine app will also be part of the Android 4.1 Jelly Bean OTA that'll be sent to ICS-running Nexus devices around the same time. An email sent to currents partners today indicate that Currents' user base is set to increase significantly as it goes from an optional app on the Play Store to a part of the core collection of Google apps. As such, we'd also expect to see Currents shipping out on future Google-certified Jelly Bean devices, as it becomes a more established member of the Google apps family.

If you're using Currents, be sure to add Android Central to your library today.

Source: http://feedproxy.google.com/~r/androidcentral/~3/hNk9_PumAC4/story01.htm

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Top women's secret to success: Title IX

La Salle University

Jennifer Ngo, now a special agent for the FBI, during her playing days at La Salle University

By Eve Tahmincioglu

Jennifer Ngo, 32, a special agent for the FBI, played basketball when she was in college. Elyse Darefsky, 54, an IT manager at Cigna, was a big collegiate volleyball and basketball player. And Sarah Ann Slater, 23, who starts graduate school at the London School of Economics in the fall, was a junior tennis champ.

All three women credit sports for their achievements beyond the playing field, and studies show playing sports in your youth can indeed contribute to future career success.

?For me, it was about being part of something bigger than yourself,? said Ngo, who also played soccer growing up. ?As I got older, it helped me with my career.?

Their experiences point to how important it is for girls to have opportunities in athletics.?They also?underscore the significance of Title IX, which paved the way for more gender equity in high school and college sports, and celebrates its 40^th anniversary this month.

Engaging in sports in youth can help women, and men, attain career success later in life, and many prominent women often point to that experience as a reason for their ability to climb the ladder.

Irene Rosenfeld, CEO of Kraft Foods, played everything from field hockey to basketball when she was in high school; former Alaska governor and vice-presidential candidate Sarah Palin touted her sporting past as a basketball player for the Wasilla Warriors; and SEC Chairman Mary Schapiro was a lacrosse player in college.

As Title IX celebrates its 40th anniversary, hear from three women who've seen the battle from all sides.

Of her lacrosse background at Franklin & Marshall College, where she captained the first varsity team in 1977, Schapiro told Lacrosse Magazine in the March issue, that the game helped her professional life.

?Lacrosse is truly a team endeavor,? she said. ?You have work together, you have to be constantly mindful of where your teammates are, you have to be willing to be in the supporting role, you have to be able to read signals and be prepared to regroup ? all of these are important to workplace success. It also taught me to take risks.?

According to a report by research firm Catalyst published in May, 82 percent of women executives played organized sports after elementary school, and nearly 60 percent said it gave them ?a competitive edge over others in the business world.??

Learning how to compete is among the top life skills youth sport members gain from their participation, according to research from Boston University?s School of Education published last year.

There is ?a direct transfer of life skills from sport to work,? found the research titled ?Career Success and Life Skill Development Through Sports,? which was part of a doctoral thesis by Gavin Bruce Barton.

He found that besides competitiveness, sports participation also developed an individual?s work ethic, ability to handle pressure, resilience, teamwork and confidence.

Surprisingly, the study also found, that ?sport participation as a source of life skill development was cited far more frequently than family, work or education.?

And, the author added, ?Life skills developed in sport can contribute to later work success.?

You don?t have to tell Cigna?s Darefsky?s that. ?I learned more playing sports than I did in school,? she explained. ?I was an introvert, and the confidence that you gain playing sports, you can?t measure that.?

She recalled going on her first job interview at Cigna in her final year of college, right after her basketball team at Clark University had a huge win over Dartmouth. ?It gave me a sense of confidence,? she said, allowing her to nail the interview.

Slater, the recent grad who played tennis, also has seen the benefits.

Courtesy of Sarah Ann Slater

?Being a part of sports actively in my youth and throughout my adolescence really taught me a lot about discipline, time management, and taking responsibility for myself and my own successes or failures,? she said. ?Even though I am not active in competitive sports any more I?was able to successfully transfer those skills into other arenas of my life, mainly academics as a college student, and they continue to be a part of all decisions I make as I go forward with my life.?

Clearly, youth sports can be an ultimate career boon, and Title IX has opened the door for?many women to participate and then reap the future benefits, said Marilyn Strawbridge, professor of physical education at Butler University in Indianapolis, who has studied the impact of sports on women.

Despite the law?s success, however, we have a long way to go when it comes to ensuring more girls get some serious locker room time, an experience that will only help them as they go out into the work world.

?Title IX has been wonderful but there?s still parity to be reached,? stressed Strawbridge. ?Unfortunately we?re still seeing lower rates of sports participation by girls in high school and college and they still get a smaller part of the athletics dollar.?

And that?s a problem given the payoff sports engagement offers women later in their careers and in their lives overall, she pointed out.

Live Poll

Do you think that girls? (and boys?) participation in sports can help lead to greater success later in life?

• 186770

  Yes

  89%

• 186771

  No

  9%

• 186772

  Not sure

  3%

VoteTotal Votes: 35

?Women in sports are better equipped to view themselves as equals; they know how to compete and put themselves out there, and take risks for something better,? she explained. ?They live with consequence and are healthier individuals all the way around, mentally and physically.?

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Tennis legend Billie Jean King has been a tireless advocate for Title IX both before and since its passage. She reflects on her career and the landmark legislation.

?

Source: http://lifeinc.today.msnbc.msn.com/_news/2012/06/29/12459328-playing-youth-sports-helps-women-in-their-careers?lite

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